Exhibit 32.1

Certification of

Principal Executive Officer

Pursuant to Section 906 of the Sarbanes-Oxley Act of 2002

I, Lucy Lu, M.D., Chief Executive Officer of Avenue Therapeutics, Inc. (the “Company”), in compliance with Section 906 of the Sarbanes-Oxley Act of 2002, hereby certify that, to the best of my knowledge, the Company’s Quarterly Report on Form 10-Q for the period ended March 31, 2021 (the “Report”) filed with the Securities and Exchange Commission:

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Fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934; and

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The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of the Company.

	/s/ Lucy Lu, M.D.
	Lucy Lu, M.D.
	President, Chief Executive Officer and Director
	(Principal Executive Officer)
	May 17, 2021